Crystallization and preliminary crystallographic analysis of earthworm fibrinolytic enzyme component B from Eisenia fetida

Authors: Wang, Feng; Wang, Chao; Li, Mei; Gui, Lulu; Zhang, Jiping; Chang, Wenrui

Source: Acta Crystallographica Section D, Volume 60, Number 5, May 2004 , pp. 933-935(3)

Publisher: Blackwell Publishing

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Abstract:

Earthworm fibrinolytic enzyme component B (EFE-b) is one of three high-fibrinolytic components found in Eisenia fetida. Several crystal forms were obtained by the hanging-drop vapour-diffusion technique. Diffraction data were collected from two well diffracting crystal forms. Crystal form I diffracted to a resolution of 2.25 Å but was merohedrally twinned, with space group P2₁3, unit-cell parameters a = b = c = 122.9 Å and two molecules per asymmetric unit. Crystal form II diffracted to beyond 2.06 Å resolution and belonged to space group P6₃22, with unit-cell parameters a = b = 96.4, c = 150.8 Å and one molecule per asymmetric unit. Crystal form II was a true single crystal and is suitable for structure determination.

Keywords: earthworm fibrinolytic enzyme; merohedral twinning

Document Type: Research article

DOI: 10.1107/S0907444904004895

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