Crystallization and preliminary crystallographic analysis of the Escherichia coli water channel AqpZ

Authors: Daniels, Brenda V.; Jiang, Jian-Sheng; Fu, Dax

Source: Acta Crystallographica Section D, Volume 60, Number 3, March 2004 , pp. 561-563(3)

Publisher: Blackwell Publishing

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Abstract:

AqpZ is a 24 kDa integral membrane protein that facilitates water movement across the plasma membrane of Escherichia coli. In this study, the first crystallization and preliminary X-ray analysis of AqpZ are described. AqpZ was overexpressed and purified with a yield of 13 mg of purified AqpZ per litre of cell culture. The purified AqpZ was shown to be a monodisperse species consisting of tetrameric protein-detergent complexes. A crystallization condition for producing diffraction-quality crystals was identified. Initial X-ray analysis indicated that the diffraction limit of AqpZ extended to 3.6 Å. Crystals were found to belong to space groups P4₁22 or P4₃22, with unit-cell parameters a = b = 119.04, c = 380.23 Å.

Keywords: AqpZ; water channels

Document Type: Research article

DOI: 10.1107/S090744490302972X

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