Crystallization and preliminary X-ray diffraction analysis of Wza outer-membrane lipoprotein from Escherichia coli serotype O9a:K30

Authors: Beis, Konstantinos; Nesper, Jutta; Whitfield, Chris; Naismith, James H.

Source: Acta Crystallographica Section D, Volume 60, Number 3, March 2004 , pp. 558-560(3)

Publisher: Blackwell Publishing

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Abstract:

A novel integral membrane lipoprotein, Wza, from Escherichia coli serotype O9a:K30 has been purified and crystallized. Wza is required for the surface expression of the serotype K30 group 1 capsular polysaccharide of E. coli; closely related homologues are found in other bacteria that produce extracellular polysaccharides. The Wza crystals belong to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a = 94.6, b = 215.5, c = 218.5 Å. A data set to 3.0 Å with 99.8% completeness and an R_merge of 10.5% has been collected from a single crystal.

Keywords: outer-membrane lipoproteins; Wza

Document Type: Research article

DOI: 10.1107/S0907444903029494

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