Crystallization and preliminary X-ray diffraction study of fructan 1-exohydrolase IIa from Cichorium intybus

Authors: Verhaest, Maureen; Van den Ende, Wim; Yoshida, Midori; Le Roy, Katrien; Peeraer, Yves; Sansen, Stefaan; De Ranter, Camiel J.; Van Laere, André; Rabijns, Anja

Source: Acta Crystallographica Section D, Volume 60, Number 3, March 2004 , pp. 553-554(2)

Publisher: Blackwell Publishing

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Abstract:

Fructan 1-exohydrolase IIa (1-FEH IIa), a plant enzyme involved in fructan breakdown, has been crystallized using the hanging-drop vapour-diffusion method at 277 K. The crystals are tetragonal, belonging to space group P4₁2₁2 or P4₃2₁2, with unit-cell parameters a = 139.83, b = 139.83, c = 181.94 Å. Calculation of the Matthews coefficient indicates there to be two or three molecules in the asymmetric unit. Synchrotron radiation was used to collect a complete native data set to a resolution of 2.35 Å.

Keywords: fructan degradation; fructan exohydrolase

Document Type: Research article

DOI: 10.1107/S0907444903029317

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