Crystallization and preliminary X-ray analysis of GlcNAcα1,4Gal-releasing endo-β-galactosidase from Clostridium perfringens

Authors: Deng, Lu; Liu, Zhi-Jie; Ashida, Hisashi; Li, Su-Chen; Li, Yu-Teh; Horanyi, Peter; Tempel, Wolfram; Rose, John; Wang, Bi-Cheng

Source: Acta Crystallographica Section D, Volume 60, Number 3, March 2004 , pp. 537-538(2)

Publisher: Blackwell Publishing

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Abstract:

The unique clostridial endo-β-galactosidase (Endo-β-Gal_GnGa) capable of releasing the disaccharide GlcNAcα1,4Gal from O-glycans expressed in the gastric gland mucous cell-type mucin has been crystallized. The crystal belongs to space group P6₃, with unit-cell parameters a = 160.4, c = 86.1 Å. Under cryocooled conditions and using a synchrotron X-ray source, the crystals diffract to 1.82 Å resolution. The asymmetric unit contains two or three molecules.

Document Type: Research article

DOI: 10.1107/S0907444903028919

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