Cloning, purification, crystallization and X-ray analysis of the Escherichia coli pyrimidine nucleoside hydrolase YeiK

Authors: Giabbai, Barbara; Degano, Massimo

Source: Acta Crystallographica Section D, Volume 60, Number 3, March 2004 , pp. 524-527(4)

Publisher: Blackwell Publishing

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Abstract:

The E. coli yeiK gene product is homologous to members of the nucleoside hydrolase family of enzymes, the physiological role of which in bacteria is still unknown. Here, the cloning, expression in milligram quantities and enzymatic characterization of YeiK as a pyrimidine-specific nucleoside hydrolase is reported. Crystals of YeiK diffract X-rays to a resolution of 1.7 Å and belong to the triclinic crystal system in space group P1, with unit-cell parameters a = 44.81, b = 85.71, c = 90.68 Å, α = 112.95, β = 101.95, γ = 85.92°.

Keywords: nucleoside hydrolases; cancer gene therapy

Document Type: Research article

DOI: 10.1107/S0907444903028488

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