The structure and refinement of apocrustacyanin C2 to 1.3 Å resolution and the search for differences between this protein and the homologous apoproteins A1 and C1

Authors: Habash, Jarjis; Helliwell, John R.; Raftery, James; Cianci, Michele; Rizkallah, Pierre J.; Chayen, Naomi E.; Nneji, Gwen A.; Zagalsky, Peter F.

Source: Acta Crystallographica Section D, Volume 60, Number 3, March 2004 , pp. 493-498(6)

Publisher: Blackwell Publishing

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Abstract:

The blue carotenoprotein α-crustacyanin of Homarus gammarus lobster carapace is comprised chemically of five 20 kDa subunits. Only two genes for the proteins have been isolated (J. B. C. Findlay, personal communication) and the five apoproteins fall into two sets of homologous proteins based on their chemical properties (CRTC, consisting of apoproteins C1, C2 and A1, and CRTA, consisting of apoproteins A2 and A3). The diffraction quality of apo C2 has been improved from 2.2 to 1.3 Å and its structure solved. The structure is compared with the A1 and C1 proteins determined at 1.4 Å [Cianci et al. (2001), Acta Cryst. D57, 1219-1229] and 1.15 Å, respectively [Gordon et al. (2001), Acta Cryst. D57, 1230-1237] and found to be very similar. Normalized B-factor difference plots per residue of different types were used to try to find chemically modified residues; none were found at these resolutions. It remains possible that the differences between the CRTC proteins result from differences in amidation. By comparison of a crystal grown with glycerol (studied at 1.6 Å) and one grown without glycerol (studied at 1.3 Å) it was seen that glycerol bound at the astaxanthin site.

Keywords: apocrustacyanin C2; carotenoproteins

Document Type: Research article

DOI: 10.1107/S090744490400037X

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