Crystallization and preliminary X-ray crystallographic analysis of a ytfG gene product from Escherichia coli

Authors: Kim, In-Kwon; Yim, Hyung-Soon; Kim, Dong-Won; Kim, Young-Min; Lee, Heung-Soo; Cha, Sun-Shin; Kang, Sa-Ouk

Source: Acta Crystallographica Section D, Volume 60, Number 2, 1 February 2004 , pp. 365-367(3)

Publisher: Blackwell Publishing

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Abstract:

The Escherichia coli ytfG gene product, with NAD(P)H:quinone oxidoreductase activity, was crystallized by the hanging-drop vapour-diffusion method at 296 K. A 1.78 Å data set has been collected using synchrotron radiation at Pohang Light Source, South Korea. The crystal belongs to the primitive trigonal system, with unit-cell parameters a = b = 81.7, c = 76.8 Å. Analysis of the packing density shows that the asymmetric unit probably contains one monomer, with a solvent content of 48.8%.

Keywords: ytfG gene product

Document Type: Research article

DOI: 10.1107/S0907444903027781

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