Purification, crystallization and preliminary X-ray studies of sylvaticin, an elicitin-like protein from Pythium sylvaticum

Authors: Lascombe, Marie-Bernard; Ponchet, Michel; Cardin, Loic; Milat, Marie-Louise; Blein, Jean-Pierre; Prangé, Thierry

Source: Acta Crystallographica Section D, Volume 60, Number 2, 1 February 2004 , pp. 362-364(3)

Publisher: Blackwell Publishing

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Abstract:

Sylvaticin belongs to the elicitin family. These 10 kDa oomycetous proteins induce a hypersensitive response in plants, including necrosis and cell death, but subsequently leading to a non-specific systemic acquired resistance (SAR) against other pathogens. Sylvaticin has been crystallized using PEG 2000 MME as a precipitant agent in the presence of nickel chloride. The crystals belong to space group C2, with unit-cell parameters a = 99.29, b = 25.67, c = 67.45 Å, β = 99.66°. Diffraction data were recorded to 2.1 Å resolution at a synchrotron-radiation source.

Keywords: elicitins; sylvaticin

Document Type: Research article

DOI: 10.1107/S090744490302777X

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