Expression, purification, crystallization and preliminary crystallographic analysis of osmotically inducible protein C

Authors: Rehse, Peter H.; Nodake, Yuichi; Kuroishi, Chizu; Tahirov, Tahir H.

Source: Acta Crystallographica Section D, Volume 60, Number 2, 1 February 2004 , pp. 357-358(2)

Publisher: Blackwell Publishing

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Abstract:

Selenium-incorporated osmotically inducible protein C from the thermophilic bacterium Thermus thermophilus was overexpressed, purified and crystallized. The crystals belong to space group P1, with unit-cell parameters a = 37.58, b = 40.95, c = 48.14 Å, α = 76.93, β = 74.04, γ = 64.05°. Five data sets were collected from a single crystal to 1.6 Å using synchrotron radiation for MAD phasing. Self-rotation functions and the Matthews coefficient are consistent with two molecules in the asymmetric unit.

Keywords: OsmC; osmotic stress; Thermus thermophilus

Document Type: Research article

DOI: 10.1107/S0907444903027458

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