Crystallization and preliminary crystallographic analysis of a novel orange fluorescent protein from the Cnidaria tube anemone Cerianthus sp.

Authors: Ip, Denis T. M.; Chan, Siu-Hong; Allen, Mark D.; Bycroft, Mark; Wan, David C. C.; Wong, Kam-Bo

Source: Acta Crystallographica Section D, Volume 60, Number 2, 1 February 2004 , pp. 340-341(2)

Publisher: Blackwell Publishing

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Abstract:

A novel orange fluorescent protein, with excitation and emission maxima at 548 and 565 nm, respectively, from the Cnidaria tube anemone Cerianthus sp. has been cloned and overexpressed in Escherichia coli. The orange fluorescent protein has been crystallized by the sitting-drop vapour-diffusion method at 290 K using polyethylene glycol 3350 as a precipitant. A complete set of diffraction data was collected to 2.0 Å resolution at 100 K. The crystals belong to the space group R3, with hexagonal unit-cell parameters a = b = 216.947, c = 51.839 Å. There are four protein molecules in the asymmetric unit, giving a Matthews coefficient of 2.3 Å³ Da⁻¹ and a solvent content of 47%.

Keywords: fluorescent protein; chromophore; fluorescence resonance energy transfer

Document Type: Research article

DOI: 10.1107/S0907444903027100

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