Expression, crystallization and preliminary X-ray studies of the recombinant PTB domain of mouse dok1 protein

Authors: Shi, Ning; Liu, Yiwei; Ni, Minghao; Yang, MaoJun; Wu, Jing; Peng, Ying; Gao, Feng; Sun, Fei; Peng, Xiaozhong; Qiang, Boqin; Rao, Zihe; Yuan, Jiangang

Source: Acta Crystallographica Section D, Volume 60, Number 2, 1 February 2004 , pp. 334-336(3)

Publisher: Blackwell Publishing

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Abstract:

The PTB domain of mouse dok1 fusion protein has been overexpressed in Escherichia coli and crystallized in a form suitable for X-ray crystallographic study. Crystals have been obtained using the vapour-diffusion method and belong to space group P2₁2₁2₁. X-ray diffraction data were collected in-house to 2.5 Å resolution. A selenomethionine (SeMet) dok1 PTB fusion-protein derivative was expressed using the same expression system, purified in a reductive environment and crystals were obtained under similar conditions. Subsequently, three different wavelength data sets from the derivative crystal were collected to 2.5 Å resolution at SPring-8.

Keywords: dok1 PTB domain

Document Type: Research article

DOI: 10.1107/S0907444903026696

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