Crystallization and preliminary X-ray analysis of the helicase domains of Vasa complexed with RNA and an ATP analogue

Authors: Sengoku, Toru; Nureki, Osamu; Dohmae, Naoshi; Nakamura, Akira; Yokoyama, Shigeyuki

Source: Acta Crystallographica Section D, Volume 60, Number 2, 1 February 2004 , pp. 320-322(3)

Publisher: Blackwell Publishing

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Abstract:

The helicase fragment of Vasa was purified and its RNA-binding activity was examined by a UV cross-linking assay. The fragment was crystallized in complex with poly(U) RNA (U₁₀) and a non-hydrolyzable analogue of ATP. The crystal belonged to space group P2₁, with unit-cell parameters a = 71.06, b = 142.35, c = 130.47 Å, β = 90.86°. The cryocooled crystal diffracted to about 2.2 Å using synchrotron radiation from station BL41XU at SPring-8.

Keywords: Vasa; DEAD-box helicases

Document Type: Research article

DOI: 10.1107/S0907444903025897

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