Crystallization and preliminary crystallographic data of a leucotoxin S component from Staphylococcus aureus

Authors: Guillet, Valérie; Keller, Daniel; Prévost, Gilles; Mourey, Lionel

Source: Acta Crystallographica Section D, Volume 60, Number 2, 1 February 2004 , pp. 310-313(4)

Publisher: Blackwell Publishing

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Abstract:

Class S proteins of staphylococcal bicomponent pore-forming leucotoxins play an important role in membrane targetting and cell specificity. Wild-type and recombinant S components of the Panton-Valentine leucocidin (LukS-PV) were expressed in Staphylococcus aureus and Escherichia coli, respectively, and purified. Both proteins were crystallized in two crystal forms with Jeffamine M-600 as the precipitant at 285 K using the hanging-drop vapour-diffusion method and seeding techniques. Crystals belong to space group P2 (or P2₁) and P4₁ (or P4₃), with unit-cell parameters a = 72.3, b = 95.1, c = 108.1 Å, β = 106.4° and a = b = 94.8, c = 306.2 Å, respectively. A full set of X-ray diffraction data was collected to 2.1 Å from a single tetragonal crystal of the wild-type protein at 100 K.

Keywords: Staphylococcus aureus; pore-forming toxins; leucotoxins; S component

Document Type: Research article

DOI: 10.1107/S090744490302910X

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