IngentaConnect Increase in expression of growth hormone secretagogues receptor o...

Authors: ROH, Sang-gun¹; CHOI, Ki-choon¹; SHRESTHA, Yogendra B.¹; YOON, Chang²; Woo, Jung-Hee³; SASAKI, Shin-ichi¹

Source: Animal Science Journal, Volume 73, Number 4, August 2002 , pp. 305-308(4)

Abstract:

The mechanism by which ghrelin (endogenous growth hormone secretagogues; GHS) is involved with the differentiation of preadipocytes to adipocytes has remained largely unknown. To investigate this issue, we monitored the level of GHS receptor (GHS-R) mRNA during the differentiation of primary cultured ovine preadipocytes. The preadipocytes, which were obtained from ovine subcutaneous adipose tissues, were proliferated to confluence and then differentiated to adipocytes in differentiation medium for 10 days. The confluent preadipocytes and differentiated adipocytes at Days 3, 7 and 10 were harvested for total RNA extraction and semiquantitative reverse transcription-polymerase chain reaction analysis of GHS-R mRNA. The level of GHS-R mRNA was significantly increased during the differentiation process, whereas it was undetectable in the confluent preadipocytes. Our results demonstrate that GHS-R exists in adipocytes and ghrelin may be a new regulatory factor in the process of differentiation of ovine preadipocytes.

Keywords: adipocyte differentiation; growth hormone secretagogues receptor; preadipocytes; sheep

Document Type: Research article

DOI: 10.1046/j.1344-3941.2002.00043.x

Affiliations: 1: Faculty of Agriculture, Shinshu University, Minamiminowa-mura, Nagano-ken, Japan; 2: Department of Animal Science, Iksan College, Iksan, Korea; and 3: Laboratory of Molecular Biology, National Institute of Mental Health, Bethesda, Maryland, USA

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Increase in expression of growth hormone secretagogues receptor on differentiation of ovine preadipocytes