IngentaConnect Erythropoietin and erythropoietin-receptor producing cells demons...

Authors: Yasuda, Yoshiko¹; Okano, Masaki²; Nagao, Masaya²; Masuda, Seiji²; Fujita, Yoshihiko¹; Sasaki, Ryuzo²

Source: Anatomical Science International, Volume 77, Number 1, March 2002 , pp. 57-62(6)

Abstract:

We have previously demonstrated that mRNAs for erythropoietin and the erythropoietin receptor temporarily express on the visceral yolk sacs on days 9-11 of gestation in mice. In order to investigate the sites of expression, we performed in situ hybridization on visceral yolk sacs. Visceral yolk sacs from 10-day-old mice embryos were frozen in liquid nitrogen, and processed for cryosections. Sections were hybridized with a ³⁵S-labeled RNA probe complementary to mRNA coding for erythropoietin or erythropoietin receptor. Erythropoietin mRNA was detectable in 57.6% of the endodermal epithelial cells, while erythropoietin-receptor mRNA was discerned in 90.8% of the endodermal cells and mesodermal cells, including hemocyteblasts. Moreover, erythropoietin protein was detectable in 52.8% of the endodermal epithelial cells, and on the surface of hemocyteblasts and mesothelial cells. Erythropoietin-receptor protein was discernible in 87.2% of the endodermal cells and in the corresponding mesodermal cells to those where erythropoietin protein was expressed by immunohistochemical examinations. The results indicate that erythropoietin-synthesizing cells are located in half of the endodermal epithelial cells, while the majority of cells in the visceral yolk sac are erythropoietin-receptor-producing cells, indicating that almost all cell population in the visceral yolk sac is erythropoietin-responding cells via both autocrine and paracrine routes.

Keywords: erythropoietin mRNA; erythropoietin-receptor mRNA; in situ hybridization; mice; visceral yolk sac

Document Type: Research article

DOI: 10.1046/j.0022-7722.2002.00007.x

Affiliations: 1: Department of Anatomy (1st Division), Kinki University School of, Medicine, Osaka, and 2: Laboratory of Biosignals and Response, Graduate School of Biostudies, Kyoto University, Kyoto, Japan

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Erythropoietin and erythropoietin-receptor producing cells demonstrated by in situ hybridization in mouse visceral yolk sacs