IngentaConnect Analysis of Helicobacter pylori and nonsteroidal anti-inflammator...

Authors: Igarashi, M.¹; Takagi, A.¹; Jiang, X.²; Hasumi, K.¹; Watanabe, S.¹; Deguchi, R.¹; Miwa, T.¹

Source: Alimentary Pharmacology & Therapeutics, Volume 16, Supplement 2, April 2002 , pp. 235-239(5)

Abstract:

Background:

Helicobacter pylori and nonsteroidal anti-inflammatory drugs (NSAIDs) are important factors in gastric mucosal injury. However, the relationship between H. pylori and NSAID-related gastroduodenal mucosal injury has not been clarified. Aim:

To determine the role of H. pylori in NSAID-induced gastric mucosal injury and to examine the effects of H. pylori, indomethacin and sofalcone on gastric epithelial cells in culture, as a useful model to study gastric mucosal injury. In addition, we studied the effect of sofalcone, a gastric mucosal protection agent, on H. pylori and NSAID-induced gastric mucosal injury. Methods:

Cytotoxic and noncytotoxic strains of H. pylori were used, each with an inoculum of 10⁷ cfu/mL. The effect on the growth of RGM-1 cells (a rat gastric epithelial cell line) was studied by MTT assay, and levels of prostaglandin E₂ in culture supernatants were measured by EIA. Results:

Both cytotoxic and noncytotoxic strains of H. pylori tended to induce cell injury in RGM-1 cells at 48 h after inoculation. Indomethacin alone induced gastric epithelial injury in a dose-dependent manner, but did not augment cell injury induced by H. pylori. In addition, sofalcone (10⁻⁵ mol/L) showed a suppressive effect on indomethacin-induced gastric epithelial injury. Conclusion:

These findings indicate that indomethacin induces gastric mucosal injury regardless of H. pylori infection, and suggests that sofalcone may be a useful drug in the treatment of NSAID-induced mucosal injury.

Document Type: Research article

DOI: 10.1046/j.1365-2036.16.s2.6.x

Affiliations: 1: Division of Gastroenterology, Department of Internal Medicine, Tokai University School of Medicine, Kanagawa, Japan, 2: Beijing University, Beijing, China

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Analysis of Helicobacter pylori and nonsteroidal anti-inflammatory drug-induced gastric epithelial injury