IngentaConnect Effect of taurine on ulcerogenic response and impaired ulcer heal...

Abstract:

Background:

It is well known that neutrophil-derived hypochlorous acid interacts with ammonia (NH₄OH) to generate monochloramine (NH₂Cl) and that NH₂Cl irritates the gastric mucosa and impairs ulcer healing. Aim:

To examine the effect of taurine, a hypochlorous acid scavenger, on the mucosal ulcerogenic and the impaired healing response induced by NH₂Cl in rat stomachs, in comparison with those of methionine and glycine. Methods and results:

Under anaesthesia, oral administration of NH₂Cl (120 mmol/L) produced severe lesions in male Sprague-Dawley rat stomachs. Taurine (10-100 mg/kg) given p.o. 30 min prior to NH₂Cl dose-dependently prevented these lesions in response to NH₂Cl. This action was mimicked by methionine (3-30 mg/kg) but not by glycine (10-100 mg/kg). Under urethane anaesthesia, mucosal exposure to NH₄OH (120 mmol/L) caused a marked reduction of potential difference (PD) in the ex vivo chambered stomachs after induction of ischaemia, resulting in severe lesions. These ulcerogenic and PD responses by NH₄OH plus ischaemia were also mitigated by taurine and methionine, but not glycine, applied to the chamber 20 min before the onset of NH₄OH plus ischaemia. Moreover, oral administration of 100% ethanol produced severe haemorrhagic lesions in rat stomachs, all of which rapidly healed within 7 days after lesion induction. Daily administration of NH₂Cl (20 mmol/L) significantly delayed the healing of these lesions, but recovery of this impaired healing response was obtained by concurrent administration of taurine. Both taurine and methionine showed a potent scavenging effect against NH₂Cl in vitro. Conclusions:

(1) NH₂Cl generated either exogenously or endogenously damages the gastric mucosa and impairs the healing response; (2) taurine exerts a prophylactic effect against the deleterious effects of NH₂Cl, mainly due to its scavenging action against NH₂Cl; and (3) this effect of taurine may be useful for treatment of gastritis associated with Helicobacter pylori infection.

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Effect of taurine on ulcerogenic response and impaired ulcer healing induced by monochloramine in rat stomachs