IngentaConnect The KetySchmidt technique for repeated measurements of global cer...

Authors: Linde¹; Schmalbruch¹; Paulson¹; Madsen¹

Source: Acta Physiologica, Volume 165, Number 4, April 1999 , pp. 395-401(7)

Abstract:

Cerebral activation will increase cerebral blood flow (CBF) and cerebral glucose uptake (CMR_glc) more than it increases cerebral uptake of oxygen (CMR_O₂). To study this phenomenon, we present an application of the Kety-Schmidt technique that enables repetitive simultaneous determination of CBF, CMR_O₂, CMR_glc and CMR_lac on awake, non-stressed animals. After constant intravenous infusion with ¹³³Xenon, tracer infusion is terminated, and systemic arterial blood and cerebral venous blood are continuously withdrawn for 9 min. In this paper, we evaluate if the assumptions applied with the Kety-Schmidt technique are fulfilled with our application of the method. When measured twice in the same animal, the intra-individual variation for CBF, CMR_O₂, and CMR_glc were 10% (SD: 25%), 8% (SD: 25%), and 9% (SD: 28%), respectively. In the awake rat the values obtained for CBF, CMR_O₂ and CMR_glc were 106 mL [100 g]⁻¹ min⁻¹, 374 μmole [100 g]⁻¹ min⁻¹ and 66 μmole [100 g]⁻¹ min⁻¹, respectively. The glucose taken up by the brain during wakefulness was fully accounted for by oxidation and cerebral lactate efflux. Anaesthesia with pentobarbital induced a uniform reduction of cerebral blood flow and metabolism by ≈40%. During halothane anaesthesia CBF and CMR_glc increased by ≈50%, while CMR_O₂ was unchanged.

Keywords: activation; anaesthesia; cerebral blood flow; cerebral metabolism; conscious; Kety-Schmidt technique

Document Type: Research article

DOI: 10.1046/j.1365-201x.1999.00522.x

Affiliations: 1: Neurobiology Research Unit 9201, The Neuroscience Centre, Copenhagen University Hospital, Rigshospitalet, Denmark

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The Kety-Schmidt technique for repeated measurements of global cerebral blood flow and metabolism in the conscious rat