IngentaConnect Anticancer activity of sodium caffeate and its mechanism

Authors: XU, Feng¹; ZHANG, Sheng-hua²; SHAO, Rong-guang²; ZHEN, Yong-su²

Source: Acta Pharmacologica Sinica, Volume 26, Number 10, October 2005 , pp. 1248-1252(5)

Abstract:

Aim:

To study the anticancer activity of sodium caffeate (SC). Methods:

A nucleoside transport assay was used to analyze the inhibitory effects of SC on nucleoside rescue. The MTT assay was used to measure cell proliferation. Flow cytometry was used to measure the apoptosis of BEC-7402 induced by SC and the cell cycle distribution change. Western blotting analysis was employed to investigate Bcl-2, caspase and Bax expression. Intracellular Ca²⁺ and mitochondrial membrane potential were determined by flow cytometry. In vivo anti-tumor activity was measured using a tumor transplantation model in mice. Results:

SC inhibited the nucleoside transport of BEL-7402 cells with an IC₅₀ of 1.02 mg/mL. SC inhibited tumor cell proliferation with an IC₅₀ between 100

g/mL and 200

g/mL. SC induced BEL-7402 cell apoptosis in a time- and dose-dependent manner, which was induced by arresting cells in S phase. The in vivo study showed that tumor growth was inhibited in a dose-dependent manner. Activated caspase-3 and Bax expression were up-regulated after treatment with SC, while Bcl-2 expression was down-regulated. Intracellular Ca²⁺ was increased while mitochondrial membrane potential was decreased by SC. Conclusion:

SC is a new anticancer agent with promising potential.

Keywords: caffeic acid; anticancer activity; cell division; cell apoptosis; cell cycle; phytogenic antineoplastic agent; flow cytometry; Western blotting

Document Type: Research article

DOI: 10.1111/j.1745-7254.2005.00196.x

Affiliations: 1: Department of Pharmacology, Zhujiang Hospital, Southern Medical University, Guangzhou 510280; 2: Department of Oncology, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$50.39 plus tax Refund Policy