IngentaConnect Assessing phylogenetic relationships of Lycium samples using RAPD...

Authors: YIN, Xiao-lin¹; FANG, Kai-tai²; LIANG, Yi-zeng³; WONG, Ricky NS⁴; WYHA, Amber⁴

Source: Acta Pharmacologica Sinica, Volume 26, Number 10, October 2005 , pp. 1217-1224(8)

Abstract:

Aim:

To evaluate the phylogenetic relationships among related species of Lycium samples. Methods:

Random amplified polymorphic DNA (RAPD) fingerprinting and lab-on-a-chip electrophoresis techniques were used to analyze the characteristics of Lycium species. Seven species and 3 varieties of Lycium were studied. Based on RAPD fingerprint data obtained from 11 primers, we proposed a new index, called dispersivity, using entropy theory and projection methods to depict the diversity of the DNA fingerprints. Results:

Using the proposed dispersivity, primers were sorted and the dendrograms of the 7 species and 3 varieties of Lycium were constructed synthetically by merging primer information. Conclusion:

Phylogenetic relationships among Lycium samples were constructed synthetically based on RAPD fingerprint data generated from 11 primers.

Keywords: DNA fingerprinting; RAPD technique; lab-on-a-chip; entropy; UPGMA

Document Type: Research article

DOI: 10.1111/j.1745-7254.2005.00197.x

Affiliations: 1: School of Mathematics and Statistics, Northeast Normal University, Changchun 130024, China; 2: Department of Mathematics, Hong Kong Baptist University, Hong Kong, China; 3: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China; 4: Department of Biology, Hong Kong Baptist University, Hong Kong, China

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$50.16 plus tax Refund Policy

Assessing phylogenetic relationships of Lycium samples using RAPD and entropy theory