IngentaConnect Effects of propyl gallate on interaction between TNF-alpha and sT...

Authors: LI, Jing¹; HUANG, Jia-dong¹; WU, Bao-yan¹; CHEN, Qiang¹

Source: Acta Pharmacologica Sinica, Volume 26, Number 10, October 2005 , pp. 1212-1216(5)

Publisher: Blackwell Publishing

Abstract:

Aim:

To study the effects of propyl gallate on the interaction of tumor necrosis factor- alpha

(TNF-

) with its soluble receptor, sTNFR-I. Methods:

Interactions between TNF- alpha

and sTNFR-I were analyzed using an IAsys biosensor. sTNFR-I was immobilized on the carboxymethyl dextran (CMD) surface of the IAsys biosensor cuvettes, and TNF- alpha

preincubated with different concentrations of propyl gallate was added to the cuvettes. The resonant angle shift caused by the binding between TNF- alpha

and sTNFR-I was then recorded. Results:

sTNFR-I was immobilized on the CMD surface at a density of 2.76 ng/mm². TNF- alpha

then bound the immobilized sTNFR-I specifically, and propyl gallate was able to enhance the binding between TNF- alpha

and sTNFR-I in a dose-dependent manner. Conclusion:

The binding between TNF- alpha

and sTNFR-I is one of the targets that propyl gallate can act on in vivo. The IAsys biosensor offers a new clue as to the study on the mechanisms of action of propyl gallate.

Keywords: propyl gallate; tumor necrosis factor- alpha ; soluble tumor necrosis factor receptor; affinity biosensor

Document Type: Research article

DOI: 10.1111/j.1745-7254.2005.00198.x

Affiliations: 1: Laboratory of Biosensors, College of Life Science, Nankai University, Tianjin 300071, China

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Effects of propyl gallate on interaction between TNF- and sTNFR-I using an affinity biosensor