IngentaConnect Development of an Experimental Model of Induced Bacterial Periton...

Authors: Sánchez, Elisabet; Such, José; Teresa Chiva, Maite¹; Soriano, Germán; Llovet, Teresa²; Mercè, Javier³; Sancho, Francisco⁴; Muñoz, Carlos; Song, Xiao-yu⁵; Pérez-Mateo, Miguel; Balanzó, Joaquín; Guarner, Carlos

Source: The American Journal of Gastroenterology, Volume 102, Number 6, June 2007 , pp. 1230-1236(7)

Publisher: Blackwell Publishing

Abstract:

BACKGROUND: Spontaneous bacterial peritonitis (SBP) is a severe complication of cirrhotic patients associated with a high mortality.

AIM: To develop an available experimental model of induced bacterial peritonitis in cirrhosis.

MATERIAL Sprague-Dawley rats with carbon-tetrachloride-induced cirrhosis with (N = 22) or without (N = 101)

AND METHODS: ascites were randomized to receive an intraperitoneal administration of different concentrations of Escherichia coli (E. coli) diluted in 1 mL of sterile water in ascitic rats and in different volumes in nonascitic rats. A subgroup of nonascitic animals received ceftriaxone 4 h after E. coli inoculation. Mortality of rats was evaluated 24 h after bacterial inoculation.

RESULTS: None of the rats receiving sterile water alone and only one infected with 10⁷ cfu of E. coli died. Ascitic rats showed a lower mortality rate than nonascitic rats infected with 10⁸ or 10⁹ cfu of E. coli (P < 0.05). Mortality was higher with 10⁹ cfu than with 10⁸ cfu of E. coli in ascitic (P NS) and nonascitic (P < 0.01) rats. A trend was noted to ward higher mortality in nonascitic rats inoculated with 10⁸ cfu with increasing water volumes. A marked peritoneal polymorphonuclear cell response was observed 4 h after E. coli injection in both ascitic and nonascitic rats. Antibiotic therapy significantly reduced the mortality rate of rats infected with 10⁸ cfu (P < 0.01).

CONCLUSIONS: This experimental model of induced bacterial peritonitis in cirrhosis with or without ascites may represent a useful tool for the study of pathogenic events postinfection and for the design of new therapeutic strategies to treat patients with SBP.

(Am J Gastroenterol 2007;102:1230-1236)

Document Type: Research article

DOI: 10.1111/j.1572-0241.2007.01182.x

Affiliations: 1: Liver Unit, Hospital de la Santa Creu i Sant Pau, Autonomous University, Barcelona, Spain 2: Microbiology 3: Biochemistry 4: Pathology, Hospital de la Santa Creu i Sant Pau, Autonomous University, Barcelona, Spain 5: Research and Development Centocor, Malvern, Pennsylvania

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Development of an Experimental Model of Induced Bacterial Peritonitis in Cirrhotic Rats With or Without Ascites