IngentaConnect Bovine umbilical hernia maps to the centromeric end of Bos taurus...

Authors: Ron, M.¹; Tager-Cohen, I.¹; Feldmesser, E.¹; Ezra, E.²; Kalay, D.²; Roe, B.³; Seroussi, E.¹; Weller, J. I.¹

Source: Animal Genetics, Volume 35, Number 6, December 2004 , pp. 431-437(7)

Publisher: Blackwell Publishing

Abstract:

Summary

Twelve bull calves were produced by mating elite Israeli cows to `Glenhapton Enhancer', a Canadian Holstein bull. The frequency of umbilical hernia (UH) in the progeny of the sons ranged from 1 to 21%, consistent with the hypothesis that Enhancer is the carrier of major dominant or codominant gene with partial penetrance for UH. Five sons of Enhancer produced progeny with >10% frequency of UH including sire 3259, whereas progeny of three sons had <3% UH. A total of 116 grand-progeny of Enhancer, all progeny of 3259, were genotyped for 59 microsatellites spanning the 29 bovine autosomes. Of these offspring, 41 were affected. Significant differences in paternal allele frequencies between the affected and unaffected progeny groups were found for marker BMS1591 on bovine chromosome 8 (BTA8). The UH-associated paternal allele originated from Enhancer. The chromosomal segment associated with UH was more precisely mapped between UWCA47, on the centromeric end of BTA8 and RM321, 12 cM from the centromere. A maximum LOD score of 3.84 was obtained 2.5 cM from the centromere with a support interval of 8 cM. Haplotype analysis of eight sons of Enhancer suggested that the UH gene is located in the centromeric end of BTA8 beyond ARO71/ARO72. Thus, by integrating the results from progeny of sire 3259 and sons of Enhancer the location of the UH gene was further refined to the BTA8 segment between ARO71/ARO72 and UWCA47.

Keywords: affected individuals; autosomal dominant disorder; linkage study; microsatellite; umbilical hernia

Document Type: Research article

DOI: 10.1111/j.1365-2052.2004.01196.x

Affiliations: 1: Institute of Animal Sciences, ARO, The Volcani Center, Bet Dagan 50250, Israel 2: Israel Cattle Breeders Association, Caesaria 38900, Israel 3: Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK 73019, USA

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$50.16 plus tax Refund Policy

Bovine umbilical hernia maps to the centromeric end of Bos taurus autosome 8