IngentaConnect Characterization and mapping of bovine dopamine receptors 1 and 5

Authors: Haegeman, A.¹; Williams, J. L.²; Law, A.²; Van Zeveren, A.¹; Peelman, L. J.¹

Source: Animal Genetics, Volume 34, Number 4, August 2003 , pp. 290-293(4)

Abstract:

Summary

A cDNA encoding the bovine dopamine receptor 1 (DRD1) was isolated from a bovine cDNA library, cloned and completely sequenced. The coding region showed 93 and 91% sequence identity on DNA level and 96 and 94% on protein level with its respective porcine and human orthologs. The bovine DRD1 and dopamine receptor 5 (DRD5) were mapped, respectively, to BTA10 and 6 by radiation hybrid mapping. One SNP was found in DRD1 and four in DRD5. Using polymerase chain reaction-restriction fragment length polymorphism, 11 different European cattle breeds were screened for the presence of the DRD1 and DRD5 substitutions. Allele frequencies for DRD1 and DRD5 alleles were very similar across all the breeds examined. Allele frequency discrepancies were found between Belgian Blue beef breed and the other breeds.

Keywords: complementary deoxyribonucleic acid; dopamine receptor; mutation; polymerase chain reaction; restriction fragment length polymorphism; SSCP

Document Type: Short communication

DOI: 10.1046/j.1365-2052.2003.00994.x

Affiliations: 1: Department of Animal Nutrition, Genetics, Breeding and Ethology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. 2: Department of Genomics and Bioinformatics, Roslin Institute (Edinburgh), Roslin, Midlothian EH25 9PS, Scotland

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$50.16 plus tax Refund Policy