Analysis of polymorphic microsatellites within the bovine and ovine prion protein (PRNP) genes

Authors: Geldermann, H.¹; Preuss, S.¹; Eckert, J.¹; Han, Y.¹; Ollesch, K.¹

Source: Animal Genetics, Volume 34, Number 4, August 2003 , pp. 283-289(7)

Publisher: Blackwell Publishing

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Abstract:

Summary

Twenty-four microsatellite sites with at least three repeats were found in the bovine prion protein gene (PRNP) and 23 in the ovine PRNP gene. Eight microsatellite sites were polymorphic in cattle and six in sheep with up to 10 alleles per site. In many cases allelic DNA fragments had variants in microsatellite sites and in flanking regions. Distances between microsatellite sites in eight genes from cattle and sheep occurred on average every 0.9 kb. The numerous polymorphic microsatellite sites will improve analysis of phylogenetic origin of different PRNP alleles and trait association studies for bovine spongiform encephalopathy (BSE) and scrapie.

Keywords: cattle; DNA markers; prion protein; PRNP; sheep; VNTR (variable number of tandem repeats)

Document Type: Research article

DOI: 10.1046/j.1365-2052.2003.01006.x

Affiliations: 1: Department of Animal Breeding and Biotechnology, University of Hohenheim, Stuttgart, Germany

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