Development of 50 gene-associated microsatellite markers using BAC clones and the construction of a linkage map of swine chromosome 4
Authors: Fujishima-Kanaya, N.1; Toki, D.1; Suzuki, K.1; Sawazaki, T.1; Hiraiwa, H.; Iida, M.1; Hayashi, T.2; Uenishi, H.2; Wada, Y.3; Ito, Y.4; Awata, T.2
Source: Animal Genetics, Volume 34, Number 2, April 2003 , pp. 135-141(7)
Publisher: Blackwell Publishing
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Abstract:
Summary The development of informative polymorphic markers is essential for QTL mapping. We developed 50 microsatellite markers from BAC clones containing genes that were predicted to map swine chromosome 4 (SSC4) according to comparative analysis between human and swine chromosomes, and constructed a linkage map that consisted of 37 markers including 24 markers closely linked to genes in BAC clones. Microsatellite markers were developed by direct-sequencing of BAC clones and our results demonstrated that this method was effective for developing microsatellite markers in specific regions on chromosomes. Effective development of microsatellite markers closely linked to genes can further accelerate the comparative studies of chromosomes between different species.Keywords: BAC library; linkage map; microsatellite marker isolation; swine
Document Type: Short communication
DOI: 10.1046/j.1365-2052.2003.00967.x
Affiliations: 1: STAFF Institute, 446-1 Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki 305-0854, Japan. 2: Genome Research Department, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan. 3: Department of Agricultural Sciences, Faculty of Agriculture, Saga University, 1 Honjo, Saga 840-8502, Japan. 4: Physiology and Genetic Regulation Department, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan
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