A consolidated linkage map for rainbow trout (Oncorhynchus mykiss)

Authors: Nichols, K. M.1; Young, W. P.2; Danzmann, R. G.3; Robison, B. D.4; Rexroad, C.5; Noakes, M.6; Phillips, R. B.6; Bentzen, P.; Spies, I.7; Knudsen, K.8; Allendorf, F. W.8; Cunningham, B. M.1; Brunelli, J.1; Zhang, H.9; Ristow, S.9; Drew, R.1; Brown, K. H.1; Wheeler, P. A.1; Thorgaard, G. H.10

Source: Animal Genetics, Volume 34, Number 2, April 2003 , pp. 102-115(14)

Publisher: Blackwell Publishing

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Abstract:

Summary

Androgenetic doubled haploid progeny produced from a cross between the Oregon State University and Arlee clonal rainbow trout (Oncorhynchus mykiss) lines, used for a previous published rainbow trout map, were used to update the map with the addition of more amplified fragment length polymorphic (AFLP) markers, microsatellites, type I and allozyme markers. We have added more than 900 markers, bringing the total number to 1359 genetic markers and the sex phenotype including 799 EcoRI AFLPs, 174 PstI AFLPs, 226 microsatellites, 72 VNTR, 38 SINE markers, 29 known genes, 12 minisatellites, five RAPDs, and four allozymes. Thirty major linkage groups were identified. Synteny of linkage groups in our map with the outcrossed microsatellite map has been established for all except one linkage group in this doubled haploid cross. Putative homeologous relationships among linkage groups, resulting from the autotetraploid nature of the salmonid genome, have been revealed based on the placement of duplicated microsatellites and type I loci.

Keywords: amplified fragment length polymorphism; microsatellites; Oncorhynchus mykiss; type I markers

Document Type: Research article

DOI: 10.1046/j.1365-2052.2003.00957.x

Affiliations: 1: School of Biological Sciences, Washington State University, Pullman, WA, USA, 2: Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ, USA, 3: Deparment of Zoology, University of Guelph, Guelph, ON, Canada, 4: Department of Biology, Indiana University, Bloomington, IN, USA, 5: USDA/ARS National Center for Cool and Coldwater Aquaculture, Kearneysville, WV, USA, 6: School of Biological Sciences, Washington State University, Vancouver, WA, USA, 7: School of Aquatic and Fishery Science, University of Washington, Seattle, WA, USA, 8: Division of Biological Sciences, University of Montana, Missoula, MT, USA, 9: Department of Animal Sciences, Washington State University, Pullman, WA, USA, 10: School of Biological Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA, USA

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