IngentaConnect Sequence and transfection of BoLA-DRB3 cDNAs

Authors: Russell, G C¹; Fraser, D C¹; Craigmile, S¹; Oliver, R A¹; Dutia, B M²; Glass, E J¹

Source: Animal Genetics, Volume 31, Number 3, June 2000 , pp. 219-222(4)

Publisher: Blackwell Publishing

Key:

- Free Content

- New Content

- Subscribed Content

- Free Trial Content

Abstract:

Summary

Bovine MHC (BoLA-) DRB3 alleles encoded by the DH8A, DH22A and DH24A class II haplotypes were cloned from cDNA and characterized by sequence analysis. Comparison with other full-length DRB3 sequences suggested that DRB3 alleles may have evolved through multiple lineages. All three BoLA-DRB3 alleles were shown to express on the surface of transfected cells, and the transfectants were used to define or confirm the class II specificity of a panel of monoclonal antibodies.

Keywords: cattle; MHC genes; transfection; phenotyping

Document Type: Short communication

DOI: 10.1046/j.1365-2052.2000.00630.x

Affiliations: 1: Division Of Molecular Biology, Roslin Institute, Roslin, Midlothian EH25 9Ps, Uk 2: Department of Veterinary Pathology, University of Edinburgh, Edinburgh EH9 1QH, UK

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$50.16 plus tax Refund Policy