IngentaConnect C-terminus of TRAP in Staphylococcus can enhance the activity of ...

Authors: Yang, Guang¹; Gao, Yaping¹; Feng, Jiannan¹; Huang, Yong²; Li, Shaohua¹; Liu, Yu¹; Liu, Chuan¹; Fan, Ming¹; Shen, Beifen¹; Shao, Ningsheng

Source: Acta Biochimica et Biophysica Sinica, Volume 40, Number 5, May 2008 , pp. 452-458(7)

Publisher: Blackwell Publishing

Abstract:

In Staphylococcus aureus, the target of RNAIII activating protein (TRAP) is a membrane-associated protein whose C-terminus can be used as a vaccine to provide protection against staphylococcal infection. Here, we show for the first time by surface plasmon resonance and enzyme-linked immunosorbent assay that TRAP can specifically bind lysozyme and lysostaphin through its C-terminus (amino acids 155-167) and enhance lysozomal activities in vitro. It was also found that the traP mutant strain is more resistant to lysostaphin than wild-type. Our previous data showed that the C-terminus of TRAP might be extracellular. So our results suggested that the C-terminus of TRAP could act as the specific targeting protein of the lysozyme/lysostaphin on the S. aureus cell wall and the biological significance of the interaction might be to facilitate lysozyme/lysostaphin-mediated cell lysis.

Keywords: Staphylococcus aureus; TRAP; lysozyme; lysostaphin

Document Type: Research article

DOI: 10.1111/j.1745-7270.2008.00415.x

Affiliations: 1: Beijing Institute of Basic Medical Sciences, Beijing 100850, China 2: Department of Chemistry, Peking University, Beijing 100086, China

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$50.16 plus tax Refund Policy

C-terminus of TRAP in Staphylococcus can enhance the activity of lysozyme and lysostaphin