IngentaConnect Differential gene expression profiling of human epidermal growth ...

Authors: Wang, Yan; Peng, Haining; Zhong, Yingli; Li, Daiqiang¹; Tang, Mi¹; Ding, Xiaofeng²; Zhang, Jian

Source: Acta Biochimica et Biophysica Sinica, Volume 40, Number 5, May 2008 , pp. 397-405(9)

Abstract:

Human epidermal growth factor receptor 2 (HER2) is highly expressed in approximately 30% of breast cancer patients, and substantial evidence supports the relationship between HER2 overexpression and poor overall survival. However, the biological function of HER2 signal transduction pathways is not entirely clear. To investigate gene activation within the pathways, we screened differentially expressed genes in HER2-positive mouse mammary tumor using two-directional suppression subtractive hybridization combined with reverse dot-blotting analysis. Forty genes and expressed sequence tags related to transduction, cell proliferation/growth/apoptosis and secreted/extracellular matrix proteins were differentially expressed in HER2-positive mammary tumor tissue. Among these, 19 were already reported to be differentially expressed in mammary tumor, 11 were first identified to be differentially expressed in mammary tumor in this study but were already reported in other tumors, and 10 correlated with other cancers. These genes can facilitate the understanding of the role of HER2 signaling in breast cancer.

Keywords: mammary tumor; HER2; suppression subtractive hybridization

Document Type: Research article

DOI: 10.1111/j.1745-7270.2008.00419.x

Affiliations: 1: Department of Pathology, the Second Xiangya Hospital, Central South University, Changsha 410011, China 2: Key Laboratory of Protein Chemistry and Developmental Biology of the Education Ministry of China, College of Life Science, Hunan Normal University, Changsha 410081, China

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Differential gene expression profiling of human epidermal growth factor receptor 2-overexpressing mammary tumor