IngentaConnect Interaction between angiogenin and fibulin 1: evidence and implic...

Authors: Zhang, Hui; Gao, Xiangwei¹; Weng, Chunhua; Xu, Zhengping

Source: Acta Biochimica et Biophysica Sinica, Volume 40, Number 5, May 2008 , pp. 375-380(6)

Abstract:

Angiogenin is an angiogenic factor involved in tumorigenesis. However, the mechanism of angiogenin's action remains elusive. In the present study, we identified fibulin 1, an extracellular matrix and plasma glycoprotein, as an angiogenin-interacting molecule by yeast two-hybrid screening. This interaction was further confirmed by two different approaches. First, fibulin 1 was co-immunoprecipitated with angiogenin by anti-angiogenin monoclonal antibody in vitro, suggesting angiogenin binds with fibulin 1 directly. Then fluorescence resonance energy transfer analysis showed that fibulin 1 interacted with angiogenin in COS-7 cells, showing that the binding could occur in a cellular context. As fibulin 1 plays an important role in cell proliferation, migration, adhesion, and stabilizes new-forming blood vessel wall, the interaction between fibulin 1 and angiogenin might underline one possible mechanism of angiogenin in angiogenesis and/or tumorigenesis.

Keywords: angiogenin; fibulin 1; protein interaction; FRET; angiogenesis

Document Type: Research article

DOI: 10.1111/j.1745-7270.2008.00420.x

Affiliations: 1: Research Center for Environmental Genomics, Zhejiang University School of Medicine, Hangzhou 310058, China

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Interaction between angiogenin and fibulin 1: evidence and implication