Expression of a Buckwheat Trypsin Inhibitor Gene in Escherichia coli and its Effect on Multiple Myeloma IM-9 Cell Proliferation
Authors: ZHANG, Zheng1; LI, Yuying1; LI, Chen1; YUAN, Jingming1; WANG, Zhuanhua
Source: Acta Biochimica et Biophysica Sinica, Volume 39, Number 9, September 2007 , pp. 701-707(7)
Publisher: Blackwell Publishing
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Abstract:
The gene of buckwheat trypsin inhibitor (BTI) has been cloned and expressed in Escherichia coli. The yield of this recombinant inhibitor was over 12 mg/L by using one-step purification on a Ni2+-NTA Sepharose column. Its molecular weight was 9322.1 Da, determined by mass spectrum analysis. The MTT and cytometry analyses showed that recombinant BTI could specifically inhibit the proliferation of IM-9 human B lymphoblastoid cells (from patient with multiple myeloma) in a dose-dependent manner. The test of recombinant BTI-induced apoptosis in IM-9 cells implied that the inhibitor might have potential application in the treatment of cancer.Keywords: buckwheat trypsin inhibitor; IM-9 cell; inhibitory activity; apoptosis
Document Type: Research article
DOI: 10.1111/j.1745-7270.2007.00332.x
Affiliations: 1: Key Laboratory for Chemical Biology and Molecular Engineering of the Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China
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