Induction of Cytotoxic T Lymphocytes Specific to Malignant Glioma Using T2 Cells Pulsed with HLA-A2-restricted Interleukin-13 Receptor α2 Peptide in vitro
Authors: JIANG, Xiaobing; LU, Xiaoling; LIU, Ru'en1; ZHANG, Fangcheng2; ZHAO, Hongyang2
Source: Acta Biochimica et Biophysica Sinica, Volume 39, Number 9, September 2007 , pp. 641-648(8)
Publisher: Blackwell Publishing
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Abstract:
Interleukin-13 receptor α2 (IL-13Rα2) is a glioma-restricted cell-surface epitope not otherwise detected within the central nervous system. The present study is a report of a novel approach of targeting malignant glioma with IL-13Rα2-specific cytotoxic T lymphocyte (CTL) induced from the peripheral blood mononuclear cells of healthy donors by multiple stimulations with human leukocyte antigen (HLA)-A2-restricted IL-13Rα2345-353 peptide-pulsed T2 cells. The induced CTL showed specific lysis against T2 cells pulsed with the peptide and HLA-A2+ glioma cells expressing IL-13Rα2345-353, while HLA-A2 glioma cell lines that express IL-13Rα2345-353 could not be recognized by CTL. The peptide-specific activity was inhibited by anti-HLA class I monoclonal antibody. These results suggest that the induced CTL specific for IL-13Rα2345-353 peptide could be a potential target of specific immunotherapy for HLA-A2 patients with malignant glioma.Keywords: malignant glioma; interleukin-13 receptor α2; human leukocyte antigen-A2; cytotoxic T lymphocyte
Document Type: Research article
DOI: 10.1111/j.1745-7270.2007.00331.x
Affiliations: 1: Department of Neurosurgery, China-Japan Friendship Hospital, Beijing 100029, China 2: Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
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