IngentaConnect Different Effects of Homocysteine and Oxidized Low Density Lipopr...

Authors: HUANG, Yushan¹; PENG, Kejun²; SU, Juan¹; HUANG, Yuping³; XU, Yizhou¹; WANG, Shuren¹

Source: Acta Biochimica et Biophysica Sinica, Volume 39, Number 1, January 2007 , pp. 19-26(8)

Abstract:

We investigated the effects of homocysteine (Hcy) and oxidized low density lipoprotein (ox-LDL) on DNA methylation in the promoter region of the estrogen receptor α (ERα) gene, and its potential mechanism in the pathogenesis of atherosclerosis. Cultured smooth muscle cells (SMCs) of humans were treated by Hcy and ox-LDL with different concentrations for different periods of time. The DNA methylation status was assayed by nested methylation-specific polymerase chain reaction, the lipids that accumulated in the SMCs and foam cell formations were examined with Oil red O staining. The proliferation of SMCs was assayed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. The results showed that ox-LDL in moderate concentrations (10-40 mg/L) induced de novo methylation in the promoter region of the ERα gene of SMCs. However, high concentrations (50 mg/L) of ox-LDL, resulted in demethylation of ERα. The Hcy treatment resulted in de novo methylation in the promoter region of the ER? gene with a concentration- and treating time-dependent manner, and a dose-dependent promoting effect on SMC proliferation. These data indicated that the two risk factors for atherosclerosis had the function of inducing de novo methylation in the promoter region of the ERα gene of SMCs. However, high concentrations (50 mg/L) of ox-LDL induced demethylation, indicating that different risk factors of atherosclerosis with different potency might cause different aberrant methylation patterns in the promoter region of the ERα gene. The atherogenic mechanism of Hcy might involve the hypermethylation of the ER? gene, leading to the proliferation of SMCs in atherosclerotic lesions.

Edited by Minghua XU

Keywords: homocysteine; oxidized low density lipoprotein; estrogen receptor?; DNA methylation; atherosclerosis

Document Type: Research article

DOI: 10.1111/j.1745-7270.2007.00251.x

Affiliations: 1: Department of Pathophysiology, West China School of Preclinic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China 2: Department of Laboratory Medicine, Chengdu Medical College, Chengdu 610500, China 3: Department of Biochemistry and Molecular Biology, Gannan Medical College, Ganzhou 341000, China

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Different Effects of Homocysteine and Oxidized Low Density Lipoprotein on Methylation Status in the Promoter Region of the Estrogen Receptor α Gene