Enamel Matrix Derivative Stimulates Osteogenesis- and Chondrogenesis-related Transcription Factors in C3H10T1/2 Cells

Authors: NARUKAWA, Masataka1; SUZUKI, Naoto; TAKAYAMA, Tadahiro1; YAMASHITA, Yasuhiko2; OTSUKA, Kichibee; ITO, Koichi

Source: Acta Biochimica et Biophysica Sinica, Volume 39, Number 1, January 2007 , pp. 1-7(7)

Publisher: Blackwell Publishing

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Abstract:

Our purpose was to determine how enamel matrix derivative (EMD) affects the expression of osteogenesis- and chondrogenesis-related transcription factors in undifferentiated mesenchymal cells. C3H10T1/2 cell line, a typical pluripotential mesenchymal cell line, was cultured with or without EMD for up to 7 d. Expression of mRNAs encoding osteogenesis- and chondrogenesis-related transcription factors (Runx2, Osterix, AJ18, Dlx5, Msx2, Sox5, Sox9 and Zfp60) was measured using real-time polymerase chain reaction. Runx2 and Sox9 protein expression and the presence of bone morphogenetic protein (BMP)-6-like molecules in EMD were determined by Western blotting. EMD substantially increased mRNA levels of osteogenesis- and chondrogenesis-related transcription factors. EMD also induced Runx2 and Sox9 protein expression. Western blotting analysis of EMD using anti-BMP-6 antibody revealed immunoreactive bands corresponding to about 14 kDa and 60 kDa. These results suggest that EMD stimulates osteogenesis- and chondrogenesis-related transcription factors, and these activities may be mediated, at least in part, by BMP-6 in EMD.

Edited by Zhenming HU

Keywords: enamel matrix derivative; osteogenesis; chondrogenesis

Document Type: Research article

DOI: 10.1111/j.1745-7270.2007.00250.x

Affiliations: 1: Department of Periodontology, Nihon University School of Dentistry, Tokyo 101-8310, Japan 2: Department of Biochemistry, Nihon University School of Dentistry, Tokyo 101-8310, Japan

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