Co-localization of histamine and dopamine-
-hydroxylase in sympathetic ganglion and release of histamine from cardiac sympathetic terminals of guinea-pig
Authors: Li, M.1; Luo, X.1; Chen, L.2; Zhang, J.3; Hu, J.3; Lu, B.3
Source: Autonomic & Autacoid Pharmacology, Volume 23, Numbers 5-6, October 2003 , pp. 327-333(7)
Publisher: Blackwell Publishing
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Abstract:
Summary 1 The aim of this study was to investigate the co-localization of histamine and dopamine--hydroxylase in the superior cervical ganglion of guinea-pig and release of histamine from cardiac sympathetic terminals in guinea-pig isolated atrium. 2 Histidine decarboxylase (a histamine-synthesizing enzyme) mRNA signals were detected in the neurones of superior cervical ganglion of guinea-pig by in situ hybridization. The results of double-labelled immunofluorescence further confirmed the co-localization of histamine and dopamine--hydroxylase in the large principle neurons and small intensely fluorescent cells in the superior cervical ganglion. The immunoreactivities of both histamine and dopamine--hydroxylase were significantly attenuated after 6-hydroxydopamine-induced lesion of sympathetic nerves. 3 The refractory electrical field stimulation caused the release of histamine from cardiac sympathetic terminals of guinea-pig isolated atria (112.14 ± 40.34 ng ml-1), which was significantly attenuated to 35 ± 15.57 ng ml-1 by reserpine pretreatment. Following administering compound 48/80, a mast cell degranulator, electrical field stimulation induced a dramatic increase of endogenous histamine release from isolated atria (303.57 ±72.93 ng ml-1). When compound 48/80 was added to the reserpine-treated atria, the release of histamine induced by field stimulation was decreased to 207.14 ± 76.39 ng ml-1. 4 These results provide novel evidence that histamine co-exists with noradrenaline in sympathetic nerves and might act as a neurotransmitter to modulate sympathetic neurotransmission.
Keywords:
histamine;
histidine decarboxylase;
dopamine--hydroxylase;
superior cervical ganglion;
atrium;
guinea-pig
Document Type: Research article
DOI: 10.1111/j.1474-8673.2004.00305.x
Affiliations: 1: Department of Pharmacology 2: Institute of Neurosciences 3: Department of Histology and Embryology, The Fourth Military Medical University, Xi'an 710032, P.R. China
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