IngentaConnect p16, cyclin D1, and human telomerase reverse transcriptase expres...

Authors: Chang, Sunhee¹; Joo, Mee¹; Kim, Hanseong¹

Source: Basic and Applied Pathology, Volume 1, Number 2, June 2008 , pp. 89-92(4)

Abstract:

Background and aim: Although morphological criteria are important for diagnosis of cervical intraepithelial neoplasia, it is not possible to differentiate those that will regress from those that will progress into invasive cancer. We evaluate the usefulness of p16, cyclin D1, and human telomerase reverse transcriptase (hTERT) as diagnostic and prognostic biomarkers in cervical lesions. Methods: We studied 12 cases of benign lesion, 15 cases of low-grade squamous intraepithelial lesion, 46 cases of high-grade squamous intraepithelial lesion, and 13 cases of squamous cell carcinoma by immunohistochemistry. Results: The p16 expression was significantly correlated with high risk-human papillomavirus (HR-HPV) status, lesion grade, and decreased cyclin D1 expression. The decreased cyclin D1 expression was significantly correlated with HR-HPV status, lesion grade, and p16 expression. The positive predictive values for high grade lesion of p16 expression, HR-HPV status, and decreased cyclin D1 expression were 93.8%, 81.3%, and 51.2%, respectively. The distribution pattern of hTERT positive cells was correlated with morphologically dysplastic cells. Conclusions: A good correlation was observed between cervical lesion grade and p16 expression, decreased cyclin D1 expression, and HR-HPV status. p16 expression could be used as a diagnostic and prognostic biomarker for risk of developing cervical cancer in women infected with HPV.

Keywords: cervical neoplasm; cyclin D1; human papillomavirus; p16; telomerase

Document Type: Original article

DOI: 10.1111/j.1755-9294.2008.00019.x

Affiliations: 1: Department of Pathology, Inje University Ilsan Paik Hospital, Gyeonggi, Korea

p16, cyclin D1, and human telomerase reverse transcriptase expression in cervical premalignant and malignant lesions