IngentaConnect Chromogenic in situ hybridization detection of chromosome 7 and 1...

Authors: An, Jungsuk¹; Choi, Jungwoo¹; Shin, Bong Kyung¹; Kim, Aeree¹; Kim, Han Kyeom¹; Kim, Insun¹

Source: Basic and Applied Pathology, Volume 1, Number 2, June 2008 , pp. 66-71(6)

Abstract:

Background and aim: The chromogenic in situ hybridization (CISH) is a new method in which chromosome aberration is determined by using a chromogenic reaction. We report the aberrations of chromosome 7 and 17 in three major subtypes of renal cell carcinomas (RCC), and compared these chromosomal changes with the flow cytometric DNA ploidy patterns. Methods: Aberrations of chromosomes 7 and 17 in 21 clear cell (c), 6 papillary (p) and 15 chromophobe (ch) RCCs were studied and compared with flow cytometric DNA ploidy. Results: Two of 14 chRCCs (14.3%) were monosomic for chromosome 7, but none of the cRCCs or pRCCs were monosomic. Twelve (85.7%) chRCCs showed monosomy for chromosome 17, but none of the pRCCs were monosomic. Three pRCCs (50%), 5 of 14 (35.7%) chRCCs and 2 (9.5%) cRCCs were polysomic for chromosome 7, whereas only one pRCC was polysomic. DNA aneuploidy was found in 2 pRCCs (33.3%), 2 cRCCs (9.5%), and 6 chRCCs (46.2%). Hypodiploidy was seen in one chRCC, and triploidy was seen in all types of RCCs. DNA aneuploidy was more frequent in cases with both chromosome 7 and 17 abnormalities (80%) than in cases with abnormality of one (38.5%) or neither (4.5%). Conclusion: The CISH method is useful in detecting chromosomal change in RCC, and the identification of chromosome 17 monosomy is helpful in diagnosis of chRCC.

Keywords: chromosome 7; chromosome 17; CISH; DNA ploidy; renal cell carcinoma

Document Type: Original article

DOI: 10.1111/j.1755-9294.2008.00015.x

Affiliations: 1: Department of Pathology, Korea University Medical College, Seoul, Korea

Chromogenic in situ hybridization detection of chromosome 7 and 17 abnormalities in renal cell carcinomas and comparison to flow cytometric DNA ploidy patterns