@article {Haq:September 2005:1550-7033:313,
	author = "Haq, Furqan",
	author = "Rao, Yeswanth L.",
	author = "Keith, Charles",
	author = "Zhao, Yiping",
	author = "Zhang, Guigen",
	title = "Nano- and Micro-Structured Substrates for Neuronal Cell Development",
	journal = "Journal of Biomedical Nanotechnology",
	volume = "1",
	year = "September 2005",
	abstract = "We investigated neurite development in a rat pheochromocytoma cell line (PC12) cultured on nanostructured, micro-structured and smooth substrates. On nano-structured substrates, cells continued to proliferate and developed shorter neurites and numerous filopodia as compared with smooth substrates. On micro-structured substrates, by contrast, cells ceased to proliferate and developed multiple and longer neurites. Additionally, cells grown on nano-structured substrates did not show any increase in acetylcholinesterase activity—a measure of cell differentiation activities, whereas cells grown on micro-structured substrates did. Thus by both morphological and biochemical criteria, nano-structured substrates inhibit and micro-structured substrates enhance PC12 differentiation. These results suggest the possibility of using substrates with topographical features at the nanometer or micrometer scales to control cell growth, such as using nano-substrates to promote cell proliferation and using micro-substrates to promote cell differentiation.",
	pages = "313-319(7)",
	url = "http://www.ingentaconnect.com/content/asp/jbn/2005/00000001/00000003/art00008"
	doi = "doi:10.1166/jbn.2005.042"
}