@article {Lang:February 2002:0003-2697:225, author = "Lang Q.", author = "Cheng I.F.", author = "Wai C.M.", author = "Paszczynski A.", author = "Crawford R.L.", author = "Barnes B.", author = "Anderson T.J.", author = "Wells R.", author = "Corti G.", author = "Allenbach L.", author = "Erwin D.P.", author = "Assefi T.", author = "Mojarradi M.", title = "Supercritical Fluid Extraction and High-Performance Liquid Chromatography-Diode Array-Electrochemical Detection of Signature Redox Compounds from Sand and Soil Samples", journal = "Analytical Biochemistry", volume = "301", year = "February 2002", abstract = "

A supercritical fluid extraction procedure and a chromatographic separation/detection method were developed for the detection of Earth-based microorganisms. After microbes in a sand or a soil sample were hydrolyzed in a diluted NH4OH/acetone solution, several redox compounds from bacteria could be effectively extracted with trimethylamine-modified supercritical CO2 at 35°C and 300 atm. These signature redox-active compounds were separated by a reversed-phase HPLC column in an ion-pair mode and then monitored with a diode array detector and an electrochemical detector. The analytical results demonstrated the feasibility of using the reported techniques to detect the chemical signature of life in barren desert sand samples. ©2002 Elsevier Science (USA).

", pages = "225-234(10)", url = "http://www.ingentaconnect.com/content/ap/ab/2002/00000301/00000002/art05502" }