@article {Keskin:September 2006:1081-1206:321,
author = "Keskin, Ozlem",
author = "Birben, Esra",
author = "Sackesen, Cansin",
author = "Soyer, Ozge U.",
author = "Alyamac, Evrim",
author = "Karaaslan, Ca",
author = "Tokol, Nihan",
author = "Ercan, Hulya",
author = "Kalayci, Omer",
title = "The effect of CD14-C159T genotypes on the cytokine response to endotoxin by peripheral blood mononuclear cells from asthmatic children",
journal = "Annals of Allergy, Asthma and Immunology",
volume = "97",
year = "September 2006",
abstract = "Background: A C-T polymorphism at position 159 in the promoter of CD14 (C-159T) modulates the cellular response to endotoxin and significantly influences total IgE levels. The effect of this genetic variant on the cytokine response of the inflammatory cells is incompletely understood. Objective: To investigate the effects of CD14-C159T genotypes on the response to endotoxin by peripheral blood mononuclear cells (PBMCs) in children with asthma. Methods: The PBMCs from asthmatic children with the TT (n = 11) and CC (n = 11) genotypes at the CD14 promoter were cultured in the presence of endotoxin, 100 ng/mL; concanavalin A, 10 μg/mL; or medium alone. Concentrations of soluble CD14 (sCD14), interleukin (IL) 1β, IL-4, IL-10, IL-12, IL-13, interferon-γ, and transforming growth factor β were determined in culture supernatants by enzyme-linked immunosorbent assay, and the transcriptional differences were evaluated using reverse-transcriptase polymerase chain reaction. Results: Under unstimulated conditions, children with the TT genotype produced higher levels of sCD14 into the culture supernatant compared with children with the CC genotype (P = .03, Mann Whitney U test). Both IL-10 and IL-1β concentrations were significantly higher in culture supernatants of children with the TT genotype after endotoxin stimulation (P = .02 and P = .009, respectively, by analysis of covariance [ANCOVA]). Messenger RNA expression was consistent with the results of protein concentration for IL-10 and sCD14. Concanavalin A stimulation resulted in lower levels of IL-4 in children with the TT genotype (P = .02, ANCOVA). Conclusion: The genotype at the CD14 promoter C159T locus may significantly influence the cytokine response of PBMCs obtained from asthmatic children. Differences in IL-10 and IL-4 production by alternative genotypes may contribute to the observed genotype effect on total IgE.",
pages = "321-328(8)",
url = "http://www.ingentaconnect.com/content/acaai/aaai/2006/00000097/00000003/art00011"
}